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KMID : 0881720190340060557
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 6 p.557 ~ p.561
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Comparison of DNA Extraction Methods for the Detection of Foodborne Pathogenic Bacteria from Livestock Manure Composts
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Kim Sung-Youn
Seo Dong-Yeon
Moon Ji-Young
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Abstract
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This study investigated the efficacy of DNA extraction methods for real-time PCR detection of foodborne pathogenic bacteria in livestock manure composts. Livestock manure composts were inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus and incubated in enrichment broth. For DNA extraction, enriched samples were treated following boiling method, by chloroform, C 18 powder, and proteinase K. As a result, 4 species of bacteria were detected by real-time PCR when subjected to boiling for 30 min and treated with proteinase K. These results suggest that detection of foodborne pathogens by real-time PCR from livestock manure composts could be applicable using effective DNA extraction methodology such as the boiling method or proteinase K.
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KEYWORD
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Livestock manure composts, Real-time PCR, Boiling method, Chloroform, Proteinase K
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